mAb anti-human IFN-γ (clone MD-1)

The monoclonal antibody to human IFN-γ (clone MD-1) was first described in 1985. It has shown to neutralize the antiviral activity of both natural and recombinant human IFN-γ and in a Western Blot the antibody reacts with the 20 kDa and 25 kDa polypeptides present in natural human IFN-γ (van der Meide P.H. et al. 1985). The MD-1 antibody has been descriped as useful in a sandwich ELISA and in ELISPOT assays (Hansen I. et al. 2018, Meyaard L. et al. 1996, Shen H. et al. 2013, Wassenaar A. et al. 1995 and Wierenga E.A. et al. 1990). Further, the antibody has proven to cross react with chimpanzee and rhesus macaque IFN-γ and can be used for immunohistochemical staining of frozen tissues (Jonker M. et al. 2013 and Kap Y. et al. 2009). The MD-1 antibody has been reported for use in intracellular flow cytometric analysis (Hamann D. et al. 1996) and in Luminex systems (Giavedoni L.D. et al. 2005). Other available formats: biotin labeled (cat. no. CT218) and FITC labeled (cat. no. CT220).