Abbreviations

AEC 3-Amino-9-ethylcarbazole
APC Antigen presenting cell
ASC Antibody secreting cells
BSA Bovine serum albumin
C Celsius
CD Cluster of differentiation
CLSI Clinical and Laboratory Standards Institute
CMV Cytomegalovirus
CNS Central nerve system
ConA Concanavalin A
CSB Cytokine stabilization buffer
CTL Cytotoxic T Lymphocytes
DMSO Dimethyl sulfoxide
EBV Epstein-Barr virus
ELISA Enzyme-linked immunosorbent assay
ELISPOT Enzyme-linked immunoSPOT
EPO Erythropoietin
FCS Fetal calf serum
G Gram (unit of mass) or times gravity (x g; unit of gravity – centrifugation)
GABA Gold-labeled anti-biotin antibodies
G-CSF Granulocyte colony stimulating factor
GM-CSF Granulocyte-macrophage colony stimulating factor
H Hour(s)
HRP Horse radish peroxidase
HTLV Human T cell lymphocyte virus
ICE Influenza A, Cytomegalovirus, Epstein-Barr virus
IFN Interferon
Ig Immunoglobulin
IL Interleukin
IP Interferon gamma inducible protein
L Liter
LPS Lipopolysaccharide
MCP Monocyte chemoattractant protein
MHC Major histocompatibiliy complex
Min Minute(s)
MLR Mixed lymphocyte reaction
mRNA Messenger ribonucleic acid
MSDS Material safety data sheet
Nat natural
OD Optical density
PB Phosphate buffer
PBMC Peripheral blood mononuclear cell(s)
PBS Phosphate buffered saline
PBS-I Commercially available sterile and pyrogen free PBS
PBST PBS containing 0.05% Tween-20
PBST-B PBS containing 0.05% Tween-20 and 0.5% BSA
PHA Phytohaemagglutinin
PMA Phorbol 12-myristate 13-acetate
PPD Purified protein derivative (tuberculin)
PS Polystyrene
PVDF Polyvinylidene fluoride
Qty Quantity
Rec recombinant
RT Room temperature (temperature between 20°C and 26°C)
RT-PCR Reverse transcription polymerase chain reaction
Sec Second(s)
SFC Spot forming cell
SPP Streptavidin-HRP polymer
Std Standard dilution
STLV Simian T cell lymphocyte virus
TMB 3,3',5,5'-Tetramethylbenzidine
TNF Tumor necrosis factor
TT Tetanus toxoid
VAT Value added tax
VEGF Vascular endothelial growth factor