Catalogue Number
CT033
Description
mAb anti-rat IFN-γ biotin (DB-12) - 50 µg
Price
€ 385.00
Characteristics
clone DB-12 (biotin)
Summary
The mouse anti-rat IFN-γ monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-γ antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity and it has shown not to bind with either mouse IFN-γ nor human IFN-γ. Further, the DB-12 antibody is highly effective as coating antibody (cat. no. CT052) in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody (cat no. CT035) for detection. Also the reverse combination in which the DB-1 antibody (cat. no. CT032) functions as coating antibody and biotinylated DB-12 antibody as detector can be used in an ELISA system (Haagmans B.L. et al. 1994). The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining (Nennesmo, I. et al. 1989 and Bernard, I et al. 1998).
References list
- Flow cytometric analysis of intracellular interferon-gamma synthesis in rat CD4 T cells.
- Suppression of rat cytomegalovirus replication by antibodies against gamma interferon.
- Interferon-gamma-like immunoreactivity and T-cell marker expression in rat skeletal muscle fibres.
- Transient cellular expression of gamma-interferon in myelin-induced and T-cell line-mediated experimental autoimmune neuritis.
- Isolation and characterization of monoclonal antibodies directed to rat interferon-gamma.