mAb anti-rat IFN-γ (clone DB-12)

The mouse anti-rat IFN-γ monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-γ antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity and it has shown not to bind with either mouse IFN-γ nor human IFN-γ. Further, the DB-12 antibody is highly effective as coating antibody in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody (cat no. CT035) for detection. Also the reverse combination in which the DB-1 antibody (cat. no. CT032) functions as coating antibody and biotinylated DB-12 antibody (cat. no. CT033) as detector can be used in an ELISA system (Haagmans B.L. et al. 1994). The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining (Nennesmo, I. et al. 1989 and Bernard, I et al. 1998).