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Mouse TNF-α T cell ELISPOT kit

TNF-alpha specific spots produced by 1x10E5 mouse (CD-1) splenocytes.
Stimulation: Concanavalin A.

Catalogue Number

CT322-PR2

Description

Mouse TNF-α T cell ELISPOT kit - 2-plate

Format:

2-plate5-plate

Quantity

Add to cart

Price

€ 435.00

staining

Enzymatic

Characteristics

PVDF

Contents

• coating antibodies
• biotinylated detection antibodies
• Streptavidin-Horseradish Peroxidase
• AEC coloring system
• Blocking stock solution
• Dilution buffer R
• Tween-20
• PVDF ELISPOT plates with lids and cover slips
• detailed manual

Documentation

References list

• Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice.
  Huang, Huichang, et al. Exp Ther Med 2018 Mar;15: 3034-3039
• Novel recombinant BCG coexpressing Ag85B, ESAT-6 and Rv2608 elicits significantly enhanced cellular immune and antibody responses in C57BL/6 mice.
  Lu, Y, et al. Scand. J. Immunol. 2012 Sep;76: 271-7
• A novel method for efficient generation of antigen-specific effector T-cells using dendritic cells transduced with recombinant adeno-associated virus and p38 kinase blockade.
  Mirandola, Leonardo, et al. J Transl Med 2019 Dec 19;17: 424
• Recombinant BCG coexpressing Ag85B, ESAT-6 and Rv3620c elicits specific Th1 immune responses in C57BL/6 mice.
  Yang, Enzhuo, et al. Microb. Pathog. 2014 Apr-May;69-70: 53-9