| Year | Reference |
|---|---|
|
2013
|
Optimizing the cryopreservation of murine splenocytes for improved antigen-specific T cell function in ELISPOT
Journal for ImmunoTherapy of Cancer
2013;1: P211
|
Abstract
ELISPOT assays are routinely used to measure immune responses of T cells in fresh and frozen splenocytes pre- parations; however, standardized methods for murine ELISPOT are not widely available. Freezing cells can significantly impact the function of T cells. The aim of this study was to optimize cryopreservation protocols to retain antigen-specific T cell function at similar levels as freshly isolated T cells using murine splenocytes.
The method of cryopreservation can have a tremendous impact upon splenocytes viability and function. By using an optimized cryopreservation protocol, it is possible to obtain antigen-specific T cell function at levels similar to freshly isolated cells.
Original publication