U-CyTech has several dual-color FluoroSpot kits in its assortment were the detection of interferon-gamma is combined with one other cytokine of interest.
The dual-color FluoroSpot assay is a modification of the ELISPOT assay and is designed to detect double cytokine-secreting T cells at the single cell level. The assay is based on the use of fluorescent-labeled conjugates, which can be visualized by fluorescence microscopy or by a fluorescent immunospot image analyzer.
The FluoroSpot assay involves simultaneous staining with both Alexa 488 (green) and R-PE (red) dyes visualizing cells that produce the two cytokines of interest. Cell that release both cytokines become visible as yellow spots. The kits are supplied with PVDF membrane-bottomed (PVDF) plates, which are specially optimized for the FluoroSpot assay.
Please, click on the kit below for more details.