Cytokine measurements in marmoset in ELISA

Marmoset ELISAs

U-CyTech biosciences has developed various sensitive and specific sandwich ELISA kits for the measurement of cytokine production in the common marmoset (Callithrix jacchus).

The ELISA set-up and performance is fast and simple and suitable to quantify your marmoset cytokine of interest in cell culture supernatant, serum and/or plasma.

Our company has already developed and validated ELISA tests for several important marmoset cytokines, such as IFN-γ, TNF-α, IL-2, IL-6, IL-12/23p40, IL-13 and IL-17A.

The common marmoset is a small New World monkey, which is increasingly used as a preclinical animal model for human disease, such as multiple sclerosis (EAE model) and rheumatoid arthritis. This animal has proven to be a very important model for the evaluation of new drugs and therapies in man. Because of species-specificity, most drugs and therapies cannot be studied in lower animals such as mice and rats, but can effectively be studied in this non-human primate. Because marmosets are not aggressive in nature and much smaller than macaques, the animals have major benefits over the use of Old World monkeys including housing and the management of experimental condions. However, limiting factors of this animal model are the small quantities of blood and tissue available for immunoassays and lack of reagents to measure cellular and humoral immune responses.

Our Marmoset ELISA kits contain 96-wells plates. However for smaller size samples it is also possible to use 384-wells plate which require only 20 µl of diluted sample per well. We can recommend the 384-well plates from Greiner bio-one (cat. no. 781061).
When using a 384-well plate, we recommend to use a volume of 20 µl per well for the coating antibody solution, standard, blank, diluted samples, detector antibody solution, SPP conjugate, TMB substrate solution and Stop solution. For blocking, we recommend a volume of 50 µl per well. Please check whether your plate reader is able to read such plates.

Please do not hesitate to contact us when you have any further questions on the use of 384-well plate in our ELISAs.


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References Marmoset ELISA products 

Click on the authors for the abstract of the below mentioned acticles.

Jagessar AS, Heijmans N, Blezer ELA, Bauer J, Weissert R and 't Hart BA
Immune profile of an atypical EAE model in marmoset monkeys immunized with recombinant human myelin oligodendrocyte glycoprotein in incomplete Freund's adjuvant.
J. Neuroinflammation 12: 169 (2015)
U-CyTech products used in this study:
Marmoset IFN-γ ELISA kit
Marmoset TNF-α ELISA kit

An Overview of Models, Methods, and Reagents Developed for Translational Autoimmunity Research in the Common Marmoset (Callithrix jacchus).
Exp. anim. 62: 159-71 (2013)
U-CyTech products used in this study:
Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset. 
J. Immunol. Res. 2014: 91632 (2014) 
U-CyTech products used in this study: